TECO, reagents

Ready-to-use liquid reagent, stable until the expiration date. The kit contains high sensitivity thromboplastin 
in the presence of recombinant human tissue factor (RTF) for determination in PT citrated human plasma or
Prothrombin time, ie the time between calcium addition and fibrin formation. Because the RTF used does not
contain contaminant coagulation factors, the kit is characterized by a high sensitivity to the deficiencies 
of factors X, VII, V and II, making it suitable for estrogen-binding factor dosing: Protrombin time, in fact,
depends on the plasma concentrations of Fibrinogen (Factor I), Protrombin (Factor II), Factors V, VII, and X. 
Therefore, the test allows to measure the deficit of one of the above factors or a combination thereof citrated 
human plasma. 

The reagent was formulated to be insensitive to therapeutic levels of heparin. 

 In the PT analysis, the addition of the reagent to the patient's plasma in the presence of 
calcium ions triggers the activation of the extrinsic pathway.

This operation ends in the conversion of fibrinogen into fibrin with the formation of a clot.

It is widely used in the monitoring of oral anticoagulant therapy that is the cause of prolongation of
Prothrombin Time to reduce the activity of vitamin K acting on coagulant factors: II, VII, IX, X
as well as Protein C and S.

.

Reaction at 37 ° C. Dispense 100 ml of citrated plasma and 200 ml of PT reagent.

synthetic phospholipid

Liquid reagent, ready to use and stable until the expiration date. 
Activated partial thromboplastin time (APTT) is the time required for the formation of the clot
when you add to the citrated plasma of the sample the Synthetic Phospholipid aPTT reagent, as a 
homogeneous and standardized activator, and calcium chloride, with the aim of optimizing the activation 
of the Factor XII and thus activating the coagulative process. . The test allows to monitor the factors of the intrinsic system of 
the coagulative process: factors VIII, IX, XI and XII and factor of Fletcher. It is also largely used 
in the monitoring of heparin therapy which results in an extension of the partial thromboplastin time 
proportionally to the amount of heparin present in the sample. It is not sensitive to platelet function 
or Factor VII.
Dedicated studies have shown that the susceptibility to the concentration decrease of factor VIII, IX, XI, 
XII of the intrinsic pathway of the Synthetic Phospholipid APTT is higher than that of the Ellagic Acid 
APTT. In addition, the Synthetic Phospholipid APTT reagent is extremely sensitive to the presence of 
Lupus-type antiphospholipid antibodies (LACs). 
Reaction at 37 ° C. Dispense 100 ml of citrated plasma, 100 ml of reagent and 100 ml of calcium chloride 
(supplied separately).

exclusive expression of the amount of fibrinogen present in the sample, using the method originally described by

Clauss. In the presence of high concentrations of thrombin, the time required for the formation of the coagulant in

diluted plasma is inversely proportional to the concentration of Fibrinogen. Plasma plasma dilution with imidazole

buffer (supplied separately) serves to minimize the interference of any anticoagulants such as heparin.

Reaction at 37 ° C. Dispense 200 ml of citrated plasma and 100 ml of PT reagent.

Imidazole Buffer

To be used with FIBRINOGEN kit for pre-dilution of samples. Ready-to-use liquid reagent. Stable up to the expiration date.